VMRD Equine Serum Amyloid A Test

Early identification of infection can be challenging, as the clinical signs are often subtle. VMRD SAA is an indispensable tool to aid veterinarians in this challenge, as serum amyloid A (SAA) is rapidly responsive to clinical changes. It is virtually undetectable in normal animals but increases with acute inflammation more rapidly, dramatically, and predictably than fibrinogen or WBC count. SAA starts dropping as soon as disease begins to resolve, allowing objective monitoring of clinical condition and treatment efficacy. VMRD SAA for Equine is a 10-minute horse-side lateral flow test designed for use in field or hospital settings. Testspouches are set up for use with whole blood, with numerical results provided by the VMRD Reader. *If testing serum or plasma is desired, please contact VMRD to purchase the accessory serum/plasma measurement pack. Download Info Sheet Related research from the Journal of Equine Veterinary This product is available directly from Plasvacc. Please contact our office to order. +61 7 5463 7600 | plasmail@plasvacc.com

VMRD Feline Serum Amyloid A Test

The VMRD Feline SAA test measures serum amyloid A (SAA) in feline serum or plasma. SAA is an inflammatory biomarker that helps to diagnose infection and monitor treatment efficacy in multiple species, including cats. It is more sensitive and reliable than body temperature and WBC count for detecting acute infections, as it is highly dynamic in response to systemic inflammation. As infection resolves, SAA decreases rapidly. This 10-minute lateral flow test can easily be run in-clinic, with numerical results provided by the handheld VMRD Reader. Download Info Sheet This product is available directly from Plasvacc. Please contact our office to order. +61 7 5463 7600 | plasmail@plasvacc.com

VMRD Reader

Reader for use with VMRD point-of-care tests that provides a quantitative result in whole blood and serum samples. Download Equine Info Sheet Download Feline Info Sheet This product is available directly from Plasvacc. Please contact our office to order. +61 7 5463 7600 | plasmail@plasvacc.com

Alvedia Diagnostic Kits

Quick Test Blood Typing Canine LabTest Blood Typing Canine Quick Test Blood Typing Feline LabTest Blood Typing Feline Quick Test Blood Typing Equine Quick Test Crossmatch Canine (XM) LabTest Crossmatch Canine (XM) Quick Test Direct Antiglobulin (DAT) LabTest Direct Antigloublin (DAT) Click on the below brochure for more information Alvedia Brochure 2023

Gamma-Check®

The Gamma-Check tests provide a means for the rapid screening of immunoglobulin levels (mainly IgG) in colostrum, whole blood, or serum.  The tests are based on the ability of glutaraldehyde to react with gammaglobulin.  The occurrence of this reaction in blood, serum or colostrum causes a solid clot formation.  This clot formation is the end point of the test and the time taken for the clot to form is inversely related to the amount of gammaglobulin present.

Gamma-Check®E

The Gamma-Check-E test is designed to be a rapid screening test for foals, using whole blood or serum.  It was developed as a semi-quantitative means of measuring the foal’s IgG level.  This test offers results within 5 minutes and can be done “mare side”.  No special equipment is needed and the test can be run as early as 8 hours post-foaling allowing time for oral colostrum supplementation.  A positive result indicates that the IgG level is greater or equal to 800 mg/dl. CAUTION: False positive results are occasionally seen when samples are haemolysed or when a foal has a high fibrinogen level.  If the foal is not healthy, we do not recommend using the test.  As false negatives do occasionally occur, we do not recommend transfusing a foal with plasma based only on the results of the Gamma-Check-E test.  If the foal tests negative, we suggest repeating the test using the Foal RID test for qualitative results. Gamma Check E Instructions

Equine RID

 

Principles of Test

Single radial immunodiffusion tests are an accurate, quantitative means for measuring proteins in serum and other fluids.  The Equine RID test has been developed to allow you to measure equine IgG easily, economically, and quickly.  Serum is placed into wells in a radial immunodiffusion plate where it diffuses out into agar on the plate.  This agar contains antibodies to the IgG in equine serum.  After a few hours, because of antibody reacting with the IgG antigen, visible precipitation rings form in the agar.  The diameter of these rings is related to the IgG concentration in the serum placed into the well.  Using serum reference standards of known IgG concentration, the concentration of the IgG in a test serum can then be easily determined.

Contents of Kit

Each kit comprises: A) a radial immunodiffusion 24-well plate containing agar gel with antiserum to equine IgG dissolved in it, B) three equine reference sera of known IgG levels, C) graph paper with a measuring scale and instructions.  We do not include pipettes to measure out the 5ul test samples.  Many laboratories and clinics have their own, but we can supply boxes of 100.  These are capillary tubes with a plunger.

Performance of the Test

When the kit is used for the first time, it is necessary to use the three reference sera to construct a graph from which future readings will be taken.  This is done by allowing the plate to reach room temperature and, using the 5ul pipette, transferring 5ul of each reference sera and test samples to separate wells in the plate.  After at least 5 hours, when the rings are visible, their diameters are measured using the mm scale.  The best fit line is drawn through the three points from the reference sera and then the concentration of IgG in the test sample determined.  In subsequent tests, it is not necessary to repeat all the reference samples.  As long as one is used and the samples are left about the same amount of time, the results will be accurate if the reference chosen still lies on the graph line. The Equine kit contains standards for IgG levels of: 2560, 1400, and 280.  Used in combination with the Gamma-Check group of tests, the RID offers the ideal solution to accurately evaluate the foal with a low IgG level. Radial Immunodiffusion Test for Equine lgG